Journal: Poultry Science

Article Title: METTL14-mediated RNA m 6 A modifications of TET1 and TET2 in duck myoblast differentiation

doi: 10.1016/j.psj.2026.106435

Figure Lengend Snippet: Comparison of RNA m 6 A modification levels in duck leg muscle. (A) DPMs during the proliferation phase; (B) DPMs on day 2 of differentiation (myotubes); (C) Immunofluorescence for Desmin in DPMs during proliferation; (D) Immunofluorescence for MYHC in DPMs on day 4 of differentiation. Dot blot and quantitative analysis of m 6 A in total RNA from duck embryos at E10 and E20 are shown in panels (E) and (F); panels (G) and (H) display quantitative and dot blot analyses of m 6 A in total RNA from DPMs in the GM and DM phases. Methylene blue (MB) staining served as a control for RNA loading. E10 and E20 indicate leg muscle samples from duck embryos at 10 and 20 days of incubation, respectively. GM and DM represent undifferentiated DPMs and DPMs at 48 hours post-differentiation, respectively. * indicates P < 0.05, ** indicates P < 0.01.

Article Snippet: The membrane was blocked in 5% nonfat milk in PBS for 2 hours, followed by overnight incubation at 4°C with primary antibodies against MYHC (DSHB, Iowa, IA, USA; 0.3 μg/mL) and GAPDH (Proteintech, Wuhan, China; 1:50000).

Techniques: Comparison, Modification, Immunofluorescence, Dot Blot, Staining, Control, Incubation